Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis

doi: 10.3390/ijms23115969

Figure Lengend Snippet: Morphology of MO3.13 cells. Representative images of control cells (Ctrl) and those treated with PMA or CX-5461 taken on the 6th day of treatment. Scale bar is 20 µm.

Article Snippet: To induce differentiation, MO3.13 cells were incubated in the presence of 100 nM PMA (Sigma-Aldrich, St. Louis, MO, USA) or in the presence of 200 nM Pol I inhibitor, CX-5461 (Cayman Chemical, Ann Arbor, MI, USA) in a medium containing DMEM supplemented with 1% FBS.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis

doi: 10.3390/ijms23115969

Figure Lengend Snippet: Assessment of nucleolar integrity of MO3.13 cells. Control cells (Ctrl) and cells treated with PMA or CX-5461. Representative images after staining with specific antibodies against NPM1 (green, upper panel) and RPL6 (green, lower panel) and DAPI (cyan, both panels). Scale bar is 10 µm.

Article Snippet: To induce differentiation, MO3.13 cells were incubated in the presence of 100 nM PMA (Sigma-Aldrich, St. Louis, MO, USA) or in the presence of 200 nM Pol I inhibitor, CX-5461 (Cayman Chemical, Ann Arbor, MI, USA) in a medium containing DMEM supplemented with 1% FBS.

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis

doi: 10.3390/ijms23115969

Figure Lengend Snippet: Influence of PMA and CX-5461 on p53 activity. ( A ) Luciferase assay performed using lysates from control (Ctrl) undifferentiated MO3.13 cells and cells treated with PMA or CX-5461. Results are presented as a ratio between Firefly and Renilla luciferase activity. ( B ) A representative Western blot showing protein level of unmodified p53, total p53, and actin (loading control). ( C ) Densitometric analysis of the level of unmodified p53. Results are presented as the ratio of unmodified to total p53, both normalized to actin. ( A , C ) Statistical analysis of results ( n = 3) was performed with the use of one sample t test. Results are presented as means ± standard error. The level of statistical significance is indicated using * p ≤ 0.05, *** p ≤ 0.001.

Article Snippet: To induce differentiation, MO3.13 cells were incubated in the presence of 100 nM PMA (Sigma-Aldrich, St. Louis, MO, USA) or in the presence of 200 nM Pol I inhibitor, CX-5461 (Cayman Chemical, Ann Arbor, MI, USA) in a medium containing DMEM supplemented with 1% FBS.

Techniques: Activity Assay, Luciferase, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: RNA-Seq Transcriptome Analysis of Differentiated Human Oligodendrocytic MO3.13 Cells Shows Upregulation of Genes Involved in Myogenesis

doi: 10.3390/ijms23115969

Figure Lengend Snippet: Relative mRNA expression of selected genes in MO3.13 cells. ( A ) Expression of CASQ2 , TNNT3 , and MYH ( various isoforms ). ( B ) Expression of HSD11B1 . Control cells (Ctrl—black bars); cells treated with PMA or CX-5461, grey and dark grey bars, respectively. ( n = 3) * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: To induce differentiation, MO3.13 cells were incubated in the presence of 100 nM PMA (Sigma-Aldrich, St. Louis, MO, USA) or in the presence of 200 nM Pol I inhibitor, CX-5461 (Cayman Chemical, Ann Arbor, MI, USA) in a medium containing DMEM supplemented with 1% FBS.

Techniques: Expressing